Attomole quantitation of protein separations with accelerator mass spectrometry
نویسندگان
چکیده
منابع مشابه
Attomole level protein sequencing by Edman degradation coupled with accelerator mass spectrometry.
Edman degradation remains the primary method for determining the sequence of proteins. In this study, accelerator mass spectrometry was used to determine the N-terminal sequence of glutathione S-transferase at the attomole level with zeptomole precision using a tracer of (14)C. The transgenic transferase was labeled by growing transformed Escherichia coli on [(14)C]glucose and purified by micro...
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ژورنال
عنوان ژورنال: ELECTROPHORESIS
سال: 2001
ISSN: 0173-0835,1522-2683
DOI: 10.1002/1522-2683(200106)22:10<2037::aid-elps2037>3.0.co;2-8